Pdrive5-gfp-10

Transcript

XbaI (15) EcoRI (19) NsiI (14) SdaI (29) NotI (2) BssHII (137) SalI (304) SpeI (308) BspEI (414) HindIII (573) hAldA enh pMB1 Ori hFerL prom BspLU11I (3446) BssHII (3275) EM2K BglII (933) SmaI (3225) Zeo pDRIVE5-GFP-10 (4194 bp) BspLU11I (1000) chEF1 5'UTR SacI (1195) SmaI (1217) XhoI (1332) SV40 pAn GFP AgeI (1559) NcoI (1567) Acc65I (1789) NheI (2290) 110 NotI (2) XbaI (15) SdaI (29) NsiI (14) EcoRI (19) 1 GCGGCCGCTATGCATCTAGAATTCCTGCAGGCGAAACTCAGCTTCCTTCGTTTCCGACTTTTCCATCCGCGTCCTCCACTTCCCCGTTCCGCCCTCCCCC BssHII (137) 101 ATTGCCAACATTCTGGCTGAGTCACGGCGCCCCAGAGCGCGCCAGGCTGGGGGAAAGGAGCAGAAGGGAGGGCCCTAGCGACCCGCGGGATGTGGTCCGA 201 GTCACGTCCGAGGGGGGTGGGGAGGGATCGTGTTCTCGGCGCCCGCCCCTTCCTAGCGCGGCCTCTGGGCTGCGCCTCTCGGGGGCGGCCCGTAGCCCAG SpeI (308) SalI (304) 301 TCCGTCGACTAGTCAGGGCCCCAACCCCCCCAAGCCCCCATTTCACAACACGCTGGCGCTACAGGCGCGTGACTTCCCCTTGCTTTGGGGCGGGGGGCTG BspEI (414) 401 AGACTCCTATGTGCTCCGGATTGGTCAGGCACGGCCTTCGGCCCCGCCTCCTGCCACCGCAGATTGGCCGCTAGGCCTCCCCGAGCGCCCTGCCTCCGAG HindIII (573) 501 GGCCGGCGCACCATAAAAGAAGCCGCCCTAGCCACGTCCCCTCGCAGTTCGGCGGTCCCGCGGGTCTGTCTCAAGCT TGCCGCCAGAACACAGgtaagtg 601 ccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccatgcccctggctgcagtacgtgattcttgatc 701 ccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctgggg 801 ccgccgcgtgctaatctggtggcaccttcgcgcctgtctcgctgctttcgctaagtctctagccatttaaaatttttgataaccagctgcgacgcttttt BglII (933) BspLU11I (1000) 901 ttctggcgagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgca 1001 catgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaaactggccggcctgctctggtgcctggcctcgcgccgcc SacI (1195) 1101 gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaa SmaI (1217) 1201 tggaggacgcggcgcccgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccttcctcatccgtcgcttcatgtgactccacggagt XhoI (1332) 1301 accgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacac 1401 tgagtgggtggagactgaagagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttgcctcattctcaagc NcoI (1567) AgeI (1559) 1501 ctcagacagtggttcaaagtttttttcttccatttcagGTGTCGTGAAAACTACCCCTACCGGTCACCATGGTTTCTAAGGGAGAAGAACTCTTTACTGG 1 Me t Va l Se r Ly s G l yG l uG l uLeuPh eTh r G l 1601 TGTTGTCCCAATTCTGGTTGAGCTGGATGGTGATGTGAATGGCCACAAATTCTCTGTGTCTGGTGAAGGTGAAGGAGATGCAACTTATGGAAAGCTGACT 1 1 yVa l Va l P r o I l eLeuVa l G l uLeuAs pG l yAs pVa l As nG l yH i s Ly s Ph eSe r Va l Se r G l yG l uG l yG l uG l yAs pA l a Th r T y r G l yL y s LeuTh r 1701 45 1801 78 1901 111 2001 145 2101 178 Acc65I (1789) CTGAAGTTCATTTGTACAACAGGAAAGCTGCCAGTGCCTTGGCCAACTCTGGTGACCACCCTGACTTATGGTGTTCAATGTTTCAGCAGGTACCCTGACC LeuLy s Ph e I l eCys Th r Th r G l yL y s LeuP r oVa l P r oT r pP r oTh r LeuVa l Th r Th r LeuTh r T y r G l yVa l G l nCys Ph eSe r A r gT y r P r oAs pH ACATGAAGCAGCATGACTTCTTTAAATCTGCAATGCCAGAAGGTTATGTTCAGGAGAGGACAATCTTCTTTAAGGATGATGGAAATTATAAGACAAGGGC i s Me t Ly s G l nHi sAs pPh ePh eLy s Se r A l a Me t P r oG l uG l y T y r Va l G l nG l uAr gTh r I l ePh ePh eLysAs pAs pG l yAs nT y r Ly s Th r A r gA l AGAAGTGAAGTTTGAAGGTGATACACTGGTTAACAGAATTGAGCTGAAAGGCATTGATTTTAAGGAAGATGGAAACATTCTGGGTCACAAGCTGGAGTAC a G l uVa l Ly s Ph eG l uG l yAs pTh r LeuVa l As nAr g I l eG l uLeuLy s G l y I l eAs pPh eLy s G l uAs pG l yAs n I l eLeuG l yH i s Ly s LeuG l uT y r AACTATAATTCTCACAATGTTTACATTATGGCAGATAAGCAGAGGAATGGAATTAAGGCTAATTTCAAGATTAGACACAACATTGAGGATGGATCTGTCC As nTyrAs nSe r Hi sAs nVa l T y r I l eMe tA l aAs pLy s G l nAr gAs nG l y I l eLysA l aAs nPh eLy s I l eAr gHi sAs n I l eG l uAs pG l ySe r Va l G AACTGGCAGACCATTACCAGCAGAACACCCCTATTGGTGATGGCCCAGTTCTCCTCCCAGATAATCACTATCTCAGCACTCAATCTGCTCTGTCCAAAGA l nLeuA l aAs pHi s T y r G l nG l nAs nTh r P r o I l eG l yAs pG l y P r oVa l LeuLeuP r oAs pAs nHi s T y r LeuSe r Th r G l nSe r A l a LeuSe r LysAs NheI (2290) 2201 CCCTAATGAGAAAAGAGACCACATGGTCCTCCTGGAGTTTGTGACAGCAGCAGGAATTACTCTGGGAATGGATGAGCTGTACAAGTAAAGCTAGCTGGCC 211 pP r oAs nG l uLy s A r gAs pHi s Me t Va l LeuLeuG l uPh eVa l Th r A l aA l a G l y I l eTh r LeuG l yMe tAs pG l uLeuT y r Ly s ••• 2301 AGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT 2401 GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT 2501 AAAACCTCTACAAATGTGGTATGGAATTAATTCTAAAATACAGCATAGCAAAACTTTAACCTCCAAATCAAGCCTCTACTTGAATCCTTTTCTGAGGGAT 2601 2701 2801 2901 3001 105 3101 72 3201 38 3301 5 3401 GAATAAGGCATAGGCATCAGGGGCTGTTGCCAATGTGCATTAGCTGTTTGCAGCCTCACCTTCTTTCATGGAGTTTAAGATATAGTGTATTTTCCCAAGG TTTGAACTAGCTCTTCATTTCTTTATGTTTTAAATGCACTGACCTCCCACATTCCCTTTTTAGTAAAATATTCAGAAATAATTTAAATACATCATTGCAA TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTA ATAGAAATTGGACAGCAAGAAAGCGAGCTTCTAGCTTATCCTCAGTCCTGCTCCTCTGCCACAAAGTGCACGCAGTTGCCGGCCGGGTCGCGCAGGGCGA 125 •••As pG l nG l uG l uA l a Va l Ph eHi s Va l CysAs nG l yA l a P r oAs pAr gLeuA l a Ph ACTCCCGCCCCCACGGCTGCTCGCCGATCTCGGTCATGGCCGGCCCGGAGGCGTCCCGGAAGTTCGTGGACACGACCTCCGACCACTCGGCGTACAGCTC eG l uAr gG l y T r pP r oG l nG l uG l y I l eG l uTh r Me tA l a P r oG l ySe r A l aAs pAr gPh eAs nTh r Se r Va l Va l G l uSe r T r pG l uA l a T y r LeuG l u GTCCAGGCCGCGCACCCACACCCAGGCCAGGGTGTTGTCCGGCACCACCTGGTCCTGGACCGCGCTGATGAACAGGGTCACGTCGTCCCGGACCACACCG As pLeuG l yA r gVa l T r pVa l T r pA l a LeuTh r A s nAs pP r oVa l Va l G l nAs pG l nVa l A l a Se r I l ePh eLeuTh r Va l As pAs pAr gVa l Va l G l yA SmaI (3225) BssHII (3275) GCGAAGTCGTCCTCCACGAAGTCCCGGGAGAACCCGAGCCGGTCGGTCCAGAACTCGACCGCTCCGGCGACGTCGCGCGCGGTGAGCACCGGAACGGCAC l a Ph eAs pAs pG l uVa l Ph eAs pAr gSe r Ph eG l yL euAr gAs pTh r T r pPh eG l uVa l A l a G l yA l a Va l As pAr gA l a Th r LeuVa l P r oVa l A l a Se TGGTCAACTTGGCCATGATGGCTCCTCCTGTCAGGAGAGGAAAGAGAAGAAGGTTAGTACAATTGCTATAGTGAGTTGTATTATACTATGCAGATATACT r Th r LeuLysA l a Me t BspLU11I (3446) ATGCCAATGATTAATTGTCAAACTAGGGCTGCAGGTT AATTAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT 3501 GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG 3601 TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC 3701 AT AGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC 3801 CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT 3901 GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG 4001 TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA 4101 TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGGCTAGTTAATTAACATTTAAATC A